Comparison of Hydrogen Production Efficiency by Rhodopseudomonas palustris MP3 and Rhodopseudomonas harwoodiae SP6 Using an Iron Complex as an Enhancement Factor

In the present study, an iron(II)-nanoscale organic complex (Fe-NO) was used as enhancement factor by two different Rhodopseudomonas species of purple non-sulphur bacteria (PNSB) to produce hydrogen (H2). The Fe-NO synthesised using FeSO4·7H2O and Eucalyptus viminalis—a native Australian plant leaf extract—in a 1:2 2:1 concentration ratio. Besides, also source iron(II) for comparison with complex. photo-fermentative bacterial cultures were isolated from fishpond, only strains, MP3 SP6, found viable after several attempts quadrate streaking. After phylogenetic analysis, these strains designated R. palustris harwoodiae SP6. control, results showed that PNSBs manifested approximately 50% higher H2 yield when in fermentation broth at 10 mg/L concentration, where 10.7 ± 0.54 10.0 0.49 mL H2/L obtained respectively. study revealed could be important material efficient production.